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To ensure that hES cells are pluripotent, their ability to differentiate into all germ layers must be con rmed in vivo. Currently, the most common technique to test for the pluripotency of hES cells is through injection of ES cells in a SCID mouse [Xu et al., 2001]. Because of the lack of an immune system in these mice, the injected ES cells are not rejected and grow into tumors that can then be examined to ensure that the cells can differentiate into all germ layers. Alternatively, morphological and immunochemical assays can be performed in vitro to con rm ES cell potency. The rst step is to check the culture to ensure that the cells are morphologically similar to undifferentiated cells (i.e., tight colonies with high ratio of nucleus to cytoplasm). Human ES cells grow in compact colonies that, when undifferentiated, have a bright, even border. The colonies are rounded, with no jagged points or invaginations. The cells inside the colony should be homologous; no structures or variation should be noticeable. Colonies should be checked under a microscope before every passage. The second step is to perform immunohistochemical or immuno uorescent assays to test the expression of ES cell-speci c markers [Ling and Neben, 1997; Richards et al., 2002; Shamblott et al., 1998; Xu et al., 2001; Zandstra et al., 2000] such as stage-speci c embryonic antigens 3 and 4 (SSEA-3 and -4), Tra-1-60, Oct-4. and alkaline phosphatase (Fig. 3.1, See Color Plate 2).

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Yr. 0006 Current Assets Cash Accounts receivable Inventories (for resale) Total Current Assets Current Liabilities Accounts payable Accrued expenses payable Bank loan payable Total Current Liabilities Working Capital Net Change, Working Capital Totals

The debits and credits incurred by guests and future guests of the hotel are maintained as back of ce accounts receivable (monies owed to the hotel). Once the guest has received the goods and enjoyed the services of the hotel, then this nancial record must be transferred to the master accounts receivable for the hotel. If a guest s folio shows a debit balance (an amount the guest owes to the hotel) of $291 and the guest wants to pay that off by charging $291 to his MasterCard, then the amount is transferred to the MasterCard accounts receivable. Another type of transaction involves the back of ce accounts payable, amounts of money that have been prepaid on behalf of the guest for future consumption of goods or services (sometimes referred to as back of ce cash accounts), such as when a guest deposits a sum of money for a future stay. For example, the personal check a guest sends to the hotel, dated February 5, for a stay on December 21 must be credited rst to the

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optimistic concurrency control algorithms are discussed. As discussed before, when extending an algorithm that is designed to work on a centralized system, we have to consider the issue of control. In a centralized system, there is only one site and that site is the center of control. This is the site where transactions enter the system and where the concurrency control module of the DBMS resides. In a distributed system, on the other hand, the control may reside at a site different from the site where a transaction enters the system. The control may be centralized, residing at only one site, or distributed where multiple sites cooperate to control execution of transactions.

engineering because of their inability to withstand physiological loading. Synthetic polymers can form hydrogels with highly controlled cross-linked structures. For example, polyethylene glycol (PEG), polyvinyl alcohol, and polypropylene fumarate are synthetic polymers that have been modi ed to create cross-linked gels with controlled porosity [Drumheller and Hubbell, 1994; Behravesh et al., 2002; He et al., 2000]. Numerous methods may be used to form cross-links between polymer chains and create a hydrogel by covalent, ionic, or physical cross-links (van der Waals forces, hydrogen bonds). The polymerization or cross-linking process can be triggered by radiation, temperature changes, addition of a chemical cross-linker, or ionic agents. For many of these methods, once cross-linking is induced, the process cannot be stopped or accelerated. This lack of control of the cross-linking process and the subsequent dif culties in clinical application led to the development of photopolymerization to encapsulate cells in hydrogels for cartilage tissue engineering.